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inhibitory concentration mic microorganisms escherichia coli  (ATCC)


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    ATCC inhibitory concentration mic microorganisms escherichia coli
    Inhibitory Concentration Mic Microorganisms Escherichia Coli, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inhibitory+concentration+mic/Escherichia+coli/pm41679600-242-2-10
    Average 99 stars, based on 5672 article reviews
    inhibitory concentration mic microorganisms escherichia coli - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    In Vitro:

    Article Title: Development of cationic Pyridinium-Oxazolidinone derivatives as antibacterial agents.
    Article Snippet: Drug-resistant bacteria and biofilm-associated infections remain major challenges in anti-infective therapy, contributing to high morbidity and mortality and emphasizing the need to develop novel antimicrobial agents with a low potential to induce resistance.. Toward this, quaternization of compounds has become a viable molecular design approach for developing antibacterial agents.. In this study, we report the design, synthesis, and antibacterial evaluation of a series of novel ring-C-modified quaternary ammonium oxazolidinone derivatives bearing various lipophilic substituents (alkyland aryl-moieties).

    Activity Assay:

    Article Title: Development of cationic Pyridinium-Oxazolidinone derivatives as antibacterial agents.
    Article Snippet: Drug-resistant bacteria and biofilm-associated infections remain major challenges in anti-infective therapy, contributing to high morbidity and mortality and emphasizing the need to develop novel antimicrobial agents with a low potential to induce resistance.. Toward this, quaternization of compounds has become a viable molecular design approach for developing antibacterial agents.. In this study, we report the design, synthesis, and antibacterial evaluation of a series of novel ring-C-modified quaternary ammonium oxazolidinone derivatives bearing various lipophilic substituents (alkyland aryl-moieties).

    Article Title: Fabaceae Flavonoids Beyond the Commonplace: A Review of Chemical Diversity, Pharmacological Activities, Mass Spectrometric Profiling and In Silico Insights into Their Subclasses.
    Article Snippet: .. Among them, Vouacapan (100) exhibited notable antifungal activity, with a minimum inhibitory concentration (MIC) of 0.98 μg/mL against Candida albicans ATCC 10231, demonstrating potency that is comparable to or even greater than that of standard antifungal agents such as fluconazole and amphotericin B (MIC 1 and 4 μg/mL, respectively) [60]. ..

    Concentration Assay:

    Article Title: Development of cationic Pyridinium-Oxazolidinone derivatives as antibacterial agents.
    Article Snippet: Drug-resistant bacteria and biofilm-associated infections remain major challenges in anti-infective therapy, contributing to high morbidity and mortality and emphasizing the need to develop novel antimicrobial agents with a low potential to induce resistance.. Toward this, quaternization of compounds has become a viable molecular design approach for developing antibacterial agents.. In this study, we report the design, synthesis, and antibacterial evaluation of a series of novel ring-C-modified quaternary ammonium oxazolidinone derivatives bearing various lipophilic substituents (alkyland aryl-moieties).

    Article Title: Isolation and antibacterial evaluation of bioactive compounds from Mussaenda philippica A.Rich.: A bioassay-guided approach
    Article Snippet: Background: Mussaenda philippica A.Rich. (commonly known as Nusa Indah in Indonesia), a member of the Rubiaceae family, has been traditionally used throughout Southern Asia to treat various ailments, including bacterial infections, inflammation, ulcers, and liver disorders.. These medicinal uses are particularly associated with its bark, leaves, sepals, and root.. Objectives: This study aimed to isolate and identify bioactive compounds from M. philippica white modified sepals using a bioassay-guided approach and evaluate their antibacterial activity.

    Article Title: Synergistic Antimicrobial Effect of Agro-Industrial Peel Extracts and Saccharomyces cerevisiae Against Listeria monocytogenes in Fruit Juice Matrices
    Article Snippet: .. According to [29], polyphenols extracted from Citrus sinensis peels demonstrate potent antibacterial properties, requiring a minimum inhibitory concentration (MIC) of 20 μg/mL to suppress L. monocytogenes ATCC 19115 and 40 μg/mL for Pseudomonas aeruginosa ATCC 27853. ..

    Article Title: Polyphenols from Byproducts: Their Applications and Health Effects
    Article Snippet: .. Similarly, another study proposed to investigate two different berry extracts, Prunus spinosa L. and Vaccinium corymbosum, and their antimicrobial activities via performing the minimum inhibitory concentration (MIC) method on Shigella flexneri ATCC 10708, Salmonella choleraesuis ATCC 12022, and E. coli ATCC 12792 [148]. .. According to results, Prunus spinosa L. extracts showed the same MIC of 250 μg/mL for E. coli ATCC 25922 and Shigella sonnei ATCC 25931, while Vaccinium corymbosum extracts showed different MIC results for Salmonella enteritidis ATCC 13076 with 2.16 mg/mL and E. coli ATCC 25922 with 3.74 mg/mL.

    Article Title: Eyes on the future: PVA-hydrogel lenses as a platform for amphotericin B ocular delivery.
    Article Snippet: .. This assertion aligns with previous reports indicating that the minimum inhibitory concentration (MIC) of AmB against Candida albicans (ATCC 90028), a common causative agent of FK, is 0.25 μg/mL [74]. ..

    Article Title: ClsDiff-AMP30: Generating Antimicrobial Peptides by a Classifier Guidance Noise Predictor
    Article Snippet: .. The minimal inhibitory concentration (MIC) of the AMPs was determined against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and methicillin-resistant S. aureus (MRSA, ATCC 43300) in accordance with the Clinical and Laboratory Standards Institute (CLSI) broth microdilution method M07–A11 [ ]. ..

    Article Title: Fabaceae Flavonoids Beyond the Commonplace: A Review of Chemical Diversity, Pharmacological Activities, Mass Spectrometric Profiling and In Silico Insights into Their Subclasses.
    Article Snippet: .. Among them, Vouacapan (100) exhibited notable antifungal activity, with a minimum inhibitory concentration (MIC) of 0.98 μg/mL against Candida albicans ATCC 10231, demonstrating potency that is comparable to or even greater than that of standard antifungal agents such as fluconazole and amphotericin B (MIC 1 and 4 μg/mL, respectively) [60]. ..

    Article Title: Exploring the antibacterial mechanism and biofilm inhibition of Plantago asiatica L. extract against Bacillus cereus with application in milk
    Article Snippet: .. In this study, its extract exhibited a minimum inhibitory concentration (MIC) of 50 μg/mL against B . cereus ATCC 11778 and demonstrated effectiveness against antibiotic-resistant strains. ..

    Membrane:

    Article Title: Development of cationic Pyridinium-Oxazolidinone derivatives as antibacterial agents.
    Article Snippet: Drug-resistant bacteria and biofilm-associated infections remain major challenges in anti-infective therapy, contributing to high morbidity and mortality and emphasizing the need to develop novel antimicrobial agents with a low potential to induce resistance.. Toward this, quaternization of compounds has become a viable molecular design approach for developing antibacterial agents.. In this study, we report the design, synthesis, and antibacterial evaluation of a series of novel ring-C-modified quaternary ammonium oxazolidinone derivatives bearing various lipophilic substituents (alkyland aryl-moieties).

    Isolation:

    Article Title: Isolation and antibacterial evaluation of bioactive compounds from Mussaenda philippica A.Rich.: A bioassay-guided approach
    Article Snippet: Background: Mussaenda philippica A.Rich. (commonly known as Nusa Indah in Indonesia), a member of the Rubiaceae family, has been traditionally used throughout Southern Asia to treat various ailments, including bacterial infections, inflammation, ulcers, and liver disorders.. These medicinal uses are particularly associated with its bark, leaves, sepals, and root.. Objectives: This study aimed to isolate and identify bioactive compounds from M. philippica white modified sepals using a bioassay-guided approach and evaluate their antibacterial activity.



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    Image Search Results


    Functional enrichment of C. albicans gene expression after treatment with iron, caspofungin, or both. Enrichment analysis of the differentially expressed genes. Only enriched categories (FDR < 0.05 and fold enrichment > 2) are represented. The size and color of the circles represent the number of genes associated with each term and FDR, respectively.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The siderophore transporter Sit1 is involved in the uptake of caspofungin by Candida albicans

    doi: 10.1128/aac.01236-25

    Figure Lengend Snippet: Functional enrichment of C. albicans gene expression after treatment with iron, caspofungin, or both. Enrichment analysis of the differentially expressed genes. Only enriched categories (FDR < 0.05 and fold enrichment > 2) are represented. The size and color of the circles represent the number of genes associated with each term and FDR, respectively.

    Article Snippet: Cell suspensions were evenly spread onto SC agar plates, and caspofungin minimal inhibitory concentration (MIC) Test Strips (Liofilchem) were placed on the center of the plate using sterile tweezers.

    Techniques: Functional Assay, Gene Expression

    Caspofungin affects iron homeostasis. ( A ) Heatmap depicting C. albicans genes related to the subcategory of iron homeostasis, which were differentially expressed under Fe, CAS, or Fe + CAS conditions. For each condition, the log 2 fold change (log 2 FC) of the selected transcripts is indicated using a color code. Genes significantly upregulated (log 2 FC > 1) or downregulated (log 2 FC < –1) in response to Fe, CAS, or Fe + CAS (treated versus untreated conditions) are shaded in red or blue, respectively. Asterisks (*) mark genes differentially expressed with FDR < 0.3 and/or log 2 CPM > 1.3 and therefore do not meet the more stringent criteria of log₂FC > 1, log 2 CPM > 3, and FDR < 0.05 and are not listed in . The complete data set is available at NCBI GEO under accession number GSE280500 . S. cerevisiae gene names were used whenever a C. albicans gene name was not assigned. ( B ) The iron content of C. albicans SC5314 cells left untreated (Control) or treated overnight with Fe, CAS, or both (Fe + CAS) was determined by ICP-AES. Significance of differences was calculated using one-way ANOVA with Tukey’s HSD post hoc test (**** P < 0.0001; n.s. not significant). ( C ) Growth of S. cerevisiae wild type (wt, BY4742), Δ ccc1 , Δ fks1, and Δ fks1ccc1 strains on SC agar plates (Control) containing Fe (5 mM), CAS (0.03 µg/mL), or Fe + CAS, for 3 days at 30°C.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The siderophore transporter Sit1 is involved in the uptake of caspofungin by Candida albicans

    doi: 10.1128/aac.01236-25

    Figure Lengend Snippet: Caspofungin affects iron homeostasis. ( A ) Heatmap depicting C. albicans genes related to the subcategory of iron homeostasis, which were differentially expressed under Fe, CAS, or Fe + CAS conditions. For each condition, the log 2 fold change (log 2 FC) of the selected transcripts is indicated using a color code. Genes significantly upregulated (log 2 FC > 1) or downregulated (log 2 FC < –1) in response to Fe, CAS, or Fe + CAS (treated versus untreated conditions) are shaded in red or blue, respectively. Asterisks (*) mark genes differentially expressed with FDR < 0.3 and/or log 2 CPM > 1.3 and therefore do not meet the more stringent criteria of log₂FC > 1, log 2 CPM > 3, and FDR < 0.05 and are not listed in . The complete data set is available at NCBI GEO under accession number GSE280500 . S. cerevisiae gene names were used whenever a C. albicans gene name was not assigned. ( B ) The iron content of C. albicans SC5314 cells left untreated (Control) or treated overnight with Fe, CAS, or both (Fe + CAS) was determined by ICP-AES. Significance of differences was calculated using one-way ANOVA with Tukey’s HSD post hoc test (**** P < 0.0001; n.s. not significant). ( C ) Growth of S. cerevisiae wild type (wt, BY4742), Δ ccc1 , Δ fks1, and Δ fks1ccc1 strains on SC agar plates (Control) containing Fe (5 mM), CAS (0.03 µg/mL), or Fe + CAS, for 3 days at 30°C.

    Article Snippet: Cell suspensions were evenly spread onto SC agar plates, and caspofungin minimal inhibitory concentration (MIC) Test Strips (Liofilchem) were placed on the center of the plate using sterile tweezers.

    Techniques: Control

    Sit1 affects caspofungin efficacy against yeast. ( A ) Structural similarity between caspofungin and cyclic hexapeptides hydroxamate siderophores. ( B ) Growth of C. albicans wt (CAF2-1) and ΔΔ sit1 mutant strains on SC agar plates (Control) containing 0.4 µM caspofungin (CAS), for 48 h or 5 days (CAS *) at 30°C. Growth of S. cerevisiae wt (YPH499) and Δ arn1-4 mutant strains on SC agar plates (Control) containing 0.1 µM caspofungin (CAS), for 48 h at 30°C. ( C ) C. albicans cells were left untreated (Control) or treated with 0.375 µg/mL caspofungin (CAS) for 3 h and plated on YPD agar plates for CFU count. Significance of differences was calculated using two-way ANOVA with Tukey’s HSD post hoc test (*** P < 0.001; * P < 0.05). ( D ) Growth of S. cerevisiae wt (YPH499) and Δ arn1-4 mutant strains transformed with a plasmid containing the CaSIT1 gene under the control of ScPGK1 promoter (p PGK-SIT1 ) or with the empty vector (v) on SC-ura agar plates (Control) containing the indicated concentrations of caspofungin (CAS), for 48 h at 30°C.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The siderophore transporter Sit1 is involved in the uptake of caspofungin by Candida albicans

    doi: 10.1128/aac.01236-25

    Figure Lengend Snippet: Sit1 affects caspofungin efficacy against yeast. ( A ) Structural similarity between caspofungin and cyclic hexapeptides hydroxamate siderophores. ( B ) Growth of C. albicans wt (CAF2-1) and ΔΔ sit1 mutant strains on SC agar plates (Control) containing 0.4 µM caspofungin (CAS), for 48 h or 5 days (CAS *) at 30°C. Growth of S. cerevisiae wt (YPH499) and Δ arn1-4 mutant strains on SC agar plates (Control) containing 0.1 µM caspofungin (CAS), for 48 h at 30°C. ( C ) C. albicans cells were left untreated (Control) or treated with 0.375 µg/mL caspofungin (CAS) for 3 h and plated on YPD agar plates for CFU count. Significance of differences was calculated using two-way ANOVA with Tukey’s HSD post hoc test (*** P < 0.001; * P < 0.05). ( D ) Growth of S. cerevisiae wt (YPH499) and Δ arn1-4 mutant strains transformed with a plasmid containing the CaSIT1 gene under the control of ScPGK1 promoter (p PGK-SIT1 ) or with the empty vector (v) on SC-ura agar plates (Control) containing the indicated concentrations of caspofungin (CAS), for 48 h at 30°C.

    Article Snippet: Cell suspensions were evenly spread onto SC agar plates, and caspofungin minimal inhibitory concentration (MIC) Test Strips (Liofilchem) were placed on the center of the plate using sterile tweezers.

    Techniques: Mutagenesis, Control, Transformation Assay, Plasmid Preparation

    Synthesis of fluorescent FAM-labeled caspofungin (CAS-FAM) (1). Reagents and conditions: (a) Boc 2 O, dioxane/H 2 O (1:1), room temperature, 48 h, 89%; (b) propargyl bromide, Cs 2 CO 3 , DMF, room temperature, 14 h, 67%; (c) fluorescein azide, 2,2′-bipyridine, CuI, sodium ascorbate, DMF, room temperature, 4 h, 77%; (d) 37% HCl, H 2 O/isopropyl alcohol (1:3, vol/vol), 2 h, room temperature, 85%.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The siderophore transporter Sit1 is involved in the uptake of caspofungin by Candida albicans

    doi: 10.1128/aac.01236-25

    Figure Lengend Snippet: Synthesis of fluorescent FAM-labeled caspofungin (CAS-FAM) (1). Reagents and conditions: (a) Boc 2 O, dioxane/H 2 O (1:1), room temperature, 48 h, 89%; (b) propargyl bromide, Cs 2 CO 3 , DMF, room temperature, 14 h, 67%; (c) fluorescein azide, 2,2′-bipyridine, CuI, sodium ascorbate, DMF, room temperature, 4 h, 77%; (d) 37% HCl, H 2 O/isopropyl alcohol (1:3, vol/vol), 2 h, room temperature, 85%.

    Article Snippet: Cell suspensions were evenly spread onto SC agar plates, and caspofungin minimal inhibitory concentration (MIC) Test Strips (Liofilchem) were placed on the center of the plate using sterile tweezers.

    Techniques: Labeling

    Sit1 mediates the uptake of CAS-FAM. ( A ) Growth of caspofungin-sensitive ( C. albicans SC5314, laboratory strain) and resistant strains ( Ca BS1, Ca BS2, and Ca 13-514) on SC agar plates containing 0.4 µM caspofungin (CAS) or 4 µM CAS-FAM, after 24 h at 30°C. ( B ) Growth of S. cerevisiae wt (YPH499) and Δ arn1-4 mutant strains transformed with a plasmid containing the CaSIT1 gene under the control of ScPGK1 promoter (p PGK-SIT1 ) or with the empty vector (v) on SC-ura agar plates (Control) containing the indicated concentrations of CAS-FAM, after 48 h at 30°C. ( C ) Quantification of the intracellular fluorescence levels of S. cerevisiae cells left untreated (Control) or treated with 1 µM CAS-FAM for 1 h. Significance of differences was calculated using two-way ANOVA with Tukey’s HSD post hoc test (**** P < 0.0001; ** P < 0.01). ( D ) Quantification of the intracellular fluorescence levels of S. cerevisiae Δ arn1-4 cells transformed with p PGK- SIT1 left untreated (Control) or treated with 1 µM CAS-FAM (CAS-FAM), 500 µM FeSO 4 (Fe), or both (Fe + CAS FAM) for 1 h was measured. Significance of differences was calculated using one-way ANOVA with Tukey’s HSD post hoc test (**** P < 0.0001; ** P < 0.01). ( E ) Fluorescence microscopy images of S. cerevisiae wt cells transformed with p PGK- SIT1 treated with 1.5 µM CAS-FAM (+ CAS FAM) for 1.5 h. Scale bar: 5 μm; arrow heads: vacuoles, BF: bright field. ( F ) Quantification of fluorescence microscopy images of cells treated with 1.5 µM CAS-FAM (CAS-FAM) for 1.5 h, either alone or in combination with 10 µM ferrichrome (CAS-FAM + FC). At least 100 cells were analyzed per condition. Significance of differences was calculated using Student’s T-test (**** P < 0.0001).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The siderophore transporter Sit1 is involved in the uptake of caspofungin by Candida albicans

    doi: 10.1128/aac.01236-25

    Figure Lengend Snippet: Sit1 mediates the uptake of CAS-FAM. ( A ) Growth of caspofungin-sensitive ( C. albicans SC5314, laboratory strain) and resistant strains ( Ca BS1, Ca BS2, and Ca 13-514) on SC agar plates containing 0.4 µM caspofungin (CAS) or 4 µM CAS-FAM, after 24 h at 30°C. ( B ) Growth of S. cerevisiae wt (YPH499) and Δ arn1-4 mutant strains transformed with a plasmid containing the CaSIT1 gene under the control of ScPGK1 promoter (p PGK-SIT1 ) or with the empty vector (v) on SC-ura agar plates (Control) containing the indicated concentrations of CAS-FAM, after 48 h at 30°C. ( C ) Quantification of the intracellular fluorescence levels of S. cerevisiae cells left untreated (Control) or treated with 1 µM CAS-FAM for 1 h. Significance of differences was calculated using two-way ANOVA with Tukey’s HSD post hoc test (**** P < 0.0001; ** P < 0.01). ( D ) Quantification of the intracellular fluorescence levels of S. cerevisiae Δ arn1-4 cells transformed with p PGK- SIT1 left untreated (Control) or treated with 1 µM CAS-FAM (CAS-FAM), 500 µM FeSO 4 (Fe), or both (Fe + CAS FAM) for 1 h was measured. Significance of differences was calculated using one-way ANOVA with Tukey’s HSD post hoc test (**** P < 0.0001; ** P < 0.01). ( E ) Fluorescence microscopy images of S. cerevisiae wt cells transformed with p PGK- SIT1 treated with 1.5 µM CAS-FAM (+ CAS FAM) for 1.5 h. Scale bar: 5 μm; arrow heads: vacuoles, BF: bright field. ( F ) Quantification of fluorescence microscopy images of cells treated with 1.5 µM CAS-FAM (CAS-FAM) for 1.5 h, either alone or in combination with 10 µM ferrichrome (CAS-FAM + FC). At least 100 cells were analyzed per condition. Significance of differences was calculated using Student’s T-test (**** P < 0.0001).

    Article Snippet: Cell suspensions were evenly spread onto SC agar plates, and caspofungin minimal inhibitory concentration (MIC) Test Strips (Liofilchem) were placed on the center of the plate using sterile tweezers.

    Techniques: Mutagenesis, Transformation Assay, Plasmid Preparation, Control, Fluorescence, Microscopy

    Sit1 is involved in the uptake of caspofungin in C. albicans . ( A ) The accumulation of caspofungin in C. albicans wt (CAF2-1) and ΔΔ sit1 cells grown in iron-depleted medium (supplemented with 300 µM of the iron chelator BPS) or iron-replete medium and treated with 1 µg/mL caspofungin for 2 h was measured by LC-MS (**** P < 0.0001; *** P < 0.001). ( B ) The iron content of C. albicans wt (CAF2-1) and ΔΔ sit1 cells left untreated (Control) or treated overnight with 5 mM FeSO 4 (Fe), 0.375 µg/mL caspofungin (CAS), or both (Fe + CAS) was determined by ICP-AES. Significance of differences was calculated using two-way ANOVA with Tukey’s HSD post hoc test (**** P < 0.0001).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The siderophore transporter Sit1 is involved in the uptake of caspofungin by Candida albicans

    doi: 10.1128/aac.01236-25

    Figure Lengend Snippet: Sit1 is involved in the uptake of caspofungin in C. albicans . ( A ) The accumulation of caspofungin in C. albicans wt (CAF2-1) and ΔΔ sit1 cells grown in iron-depleted medium (supplemented with 300 µM of the iron chelator BPS) or iron-replete medium and treated with 1 µg/mL caspofungin for 2 h was measured by LC-MS (**** P < 0.0001; *** P < 0.001). ( B ) The iron content of C. albicans wt (CAF2-1) and ΔΔ sit1 cells left untreated (Control) or treated overnight with 5 mM FeSO 4 (Fe), 0.375 µg/mL caspofungin (CAS), or both (Fe + CAS) was determined by ICP-AES. Significance of differences was calculated using two-way ANOVA with Tukey’s HSD post hoc test (**** P < 0.0001).

    Article Snippet: Cell suspensions were evenly spread onto SC agar plates, and caspofungin minimal inhibitory concentration (MIC) Test Strips (Liofilchem) were placed on the center of the plate using sterile tweezers.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Control